Genomic Characterization and Applications of Read-cloud Sequencing Technologies

In this talk, we discuss some of the advantages of read-cloud based sequencing technologies (such as the 10X Genomics System) over standard short read sequencing technologies with applications to genome re-sequencing and assembly. We present theoretical bounds for the ability of these technologies in calling variants in repeat regions of the genome, and novel ways of aligning read clouds to the reference genome and rescuing variants. We also discuss potentials of these technologies for the purpose of metagenome binning and assembly.